Premium
Adhesion of staphylococcal and Caco‐2 cells on diamond‐like carbon polymer hybrid coating
Author(s) -
Kinnari Teemu J.,
Soininen Antti,
Esteban Jaime,
Zamora Nieves,
Alakoski Esa,
Kouri VesaPetteri,
Lappalainen Reijo,
Konttinen Yrjö T.,
GomezBarrena Enrique,
Tiainen VeliMatti
Publication year - 2007
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31643
Subject(s) - materials science , adhesion , coating , staphylococcus epidermidis , titanium , diamond like carbon , substrate (aquarium) , implant , polymer , polytetrafluoroethylene , carbon fibers , composite material , biomedical engineering , chemical engineering , nanotechnology , bacteria , staphylococcus aureus , thin film , metallurgy , composite number , surgery , medicine , oceanography , biology , geology , genetics , engineering
Staphylococci cause the majority of the nosocomial implant‐related infections initiated by adhesion of planktonic bacteria to the implant surface. It was hypothesized that plasma accelerating filtered pulsed arc discharge method enables combination of the advantageous properties of diamond with the antisoiling properties of polymers. Diamond‐like carbon polytetrafluoroethylene hybrid (DLC‐PTFE‐h) coating was produced. The adhesion of S. aureus ATCC 25923 (10 8 colony‐forming units/mL) to surfaces diminished from 2.32%, 2.35%, and 2.57% of high quality DLC, titanium, and oxidized silicon, respectively, to 1.93% of DLC‐PTFE‐h. For S. epidermidis ATCC 35984 the corresponding figures were 3.90%, 3.32%, 3.47%, and 2.57%. Differences in bacterial adhesion between recombinant DLC‐PTFE‐h and other materials were statistically significant ( p < 0.05). In contrast, human Caco‐2 cells adhered as well to DLC‐PTFE‐h as to DLC, titanium, or silicon, which were all in the MTT test found to be cytocompatible. DLC‐PTFE‐h coating can be used to modify the surface properties of any surgical implants and is an unfavorable substrate for staphylococcal cells, but compatible with human Caco‐2 cells. DLC‐PTFE‐h coating may help in the combat against Staphylococcus‐ related implant infections which usually require both antibiotics and surgical removal of the implant for cure. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res 2008