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Biological activity of recombinant human growth factors released from biocompatible bone implants
Author(s) -
Ziegler Joerg,
Anger Dominique,
Krummenauer Frank,
Breitig Dieter,
Fickert Stefan,
Guenther KlausPeter
Publication year - 2008
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31625
Subject(s) - alkaline phosphatase , cell growth , recombinant dna , incubation , in vitro , basic fibroblast growth factor , bone morphogenetic protein 2 , mtt assay , bone morphogenetic protein , materials science , growth factor , cell culture , microbiology and biotechnology , biochemistry , chemistry , biology , enzyme , genetics , receptor , gene
Abstract The present investigation was performed to study the bioactivity of osteoinductive and osteoproliferative growth factors after release from biocompatible bone implants. Three types of porous carriers were used in this study: hydroxyapatite, alpha tricalcium phosphate, and a neutralized glass ceramic. Implants were loaded with recombinant human bone morphogenetic protein 2 (rh‐BMP‐2) and recombinant human basic fibroblast growth factor (rh‐bFGF) in a concentration of 2 μg/150 μL PBS each. The released growth factors were then applicated into SAOS‐2‐cell cultures. After 3, 5, and 7 days cell differentiation was measured by the activity of alkaline phosphatase (ALP), cell proliferation by using a MTT assay as well as a cell counter. Rh‐BMP‐2 released during the first hour from the scaffolds led to a significant increase of the activity of ALP in the incubated SAOS‐2‐cell culture after 3, 5, and 7 days. However, the incubation with rh‐BMP‐2 released after 24 h was not found to increase the expression of ALP. The incubation of cell cultures with rh‐bFGF released during the first hour led to a significant increase of cell number and of extinction in the MTT assay, whereas this increase was not observed after incubation with rh‐bFGF released after 24 h. The in vitro measured biological activity of released growth factors from the surface of synthetic implants is time‐depending. If prolonged osteoinductive and osteoproliferative potency of growth factors is desired, a modified application technique should be chosen to stabilize those proteins. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008