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Effects of calcium ion incorporation on osteoblast gene expression in MC3T3‐E1 cells cultured on microstructured titanium surfaces
Author(s) -
Park JinWoo,
Suh JoYoung,
Chung HyunJu
Publication year - 2008
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31618
Subject(s) - materials science , osteoblast , alkaline phosphatase , osteocalcin , osseointegration , titanium , osteopontin , calcium , bioceramic , apatite , surface modification , bone sialoprotein , chemical engineering , nanotechnology , mineralogy , metallurgy , biochemistry , chemistry , implant , biology , enzyme , in vitro , medicine , surgery , immunology , engineering
The surface characteristics of a calcium ion (Ca)‐incorporated titanium (Ti) surface, produced by hydrothermal treatment using an alkaline Ca‐containing solution, and its effects on osteoblastic differentiation were investigated. MC3T3‐E1 pre‐osteoblastic cells were cultured on machined or grit‐blasted Ti surfaces with and without Ca incorporation. The MTT assay was used to determine cell proliferation, and real‐time PCR was used for quantitative analysis of osteoblastic gene expression. Hydrothermal treatment with a Ca‐containing solution produced a crystalline CaTiO 3 nanostructure of approximately 100 nm in dimension, preserving original micron‐scaled surface topographies and microroughness caused by machining, blasting, or blasting and etching treatments. After immersion in Hank's balanced salt solution, considerable apatite formation was observed on all surfaces of the Ca‐incorporated samples. Significantly more cell proliferation was found on Ca‐incorporated Ti surfaces than on untreated Ti surfaces ( p < 0.001). Quantitative real‐time PCR analysis showed notably higher alkaline phosphatase, osteopontin, and osteocalcin mRNA levels in cells grown on Ca‐incorporated blasted surfaces than on other surfaces at an early time point. Thus, Ca incorporation may have a beneficial effect on osseointegration of microstructured Ti implants by accelerating osteoblast proliferation and differentiation during the early healing phase following implantation. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res 2008