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Preparation and immobilization of soluble eggshell membrane protein on the electrospun nanofibers to enhance cell adhesion and growth
Author(s) -
Jia Jun,
Duan YuanYuan,
Yu Jian,
Lu JianWei
Publication year - 2008
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31606
Subject(s) - nanofiber , materials science , eggshell membrane , electrospinning , contact angle , biocompatibility , membrane , scanning electron microscope , fourier transform infrared spectroscopy , chemical engineering , biomaterial , adhesion , tissue engineering , attenuated total reflection , polymer chemistry , biomedical engineering , composite material , nanotechnology , chemistry , polymer , biochemistry , medicine , engineering , metallurgy
Nonwoven poly( ε‐ caprolactone) (PCL) nanofibers were prepared by electrospinning technology, and a novel natural bioactive material, soluble eggshell membrane protein (SEP), which is made from natural eggshell membrane (ESM), was then immobilized on the nanofibers after the surface modification. The SEP‐immobilized fibrous mat was observed and characterized using scanning electron microscopy (SEM), contact angle measurement, Fourier transform infrared attenuated total reflection spectroscopy (ATR‐FTIR), X‐ray photoelectron spectroscopy (XPS), and tensile mechanical tests. Then the primary human dermal fibroblasts (HDFs) were cultured to evaluate the in vitro biocompatibility of SEP‐grafted electrospun PCL nanofibers. The results confirmed the successful immobilization of SEP on the nanofibers and also indicated that the hydrophilicity of the PCL nanofibers has been greatly improved by the SEP grafting. The results of MTT testing, SEM, and laser scanning confocal microscope (LSCM) showed that SEP immobilization can obviously enhance the attachment, spreading, and proliferation of human dermal fibroblasts (HDFs) compared with the pristine material. The SEP‐grafted PCL nanofibers can be expected to biomimic and regenerate the natural structure of eggshell membrane and to be a potential material for tissue engineering scaffold and guided tissue regeneration barrier membrane. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008