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Effects of passivation treatments on titanium alloy with nanometric scale roughness and induced changes in fibroblast initial adhesion evaluated by a cytodetacher
Author(s) -
Wang C.C.,
Hsu Y.C.,
Su F.C.,
Lu S.C.,
Lee T.M.
Publication year - 2009
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31604
Subject(s) - materials science , passivation , titanium alloy , adhesion , alloy , titanium , metallurgy , scale (ratio) , composite material , layer (electronics) , physics , quantum mechanics
Passivation treatments of titanium alloy alter not only its nanosurface characteristics of oxides and ion release but also surface roughness ( Ra ), and wettability as well, where nanosurface characteristics of oxides include chemistries of oxides, amphoteric‐OH groups adsorbed on oxides, and oxide thickness. Consequently, the passivation treatment affects the alloy's cyto‐comparability. In this study, we polish specimens to achieve nanometric scale roughness. In addition, treatment effects are evaluated for surface topology, roughness, wettability, and responses of fibroblasts consisting of MTT assay, initial adhesion strength, and morphology. The initial adhesion strength is measured using a cyto‐detacher that achieves nano‐Newton resolution. Results reveal that (1) the treatment effects on the percentage of TiOH basic groups and wettability are nearly collinear; (2) the Ra of passivated Ti‐6Al‐4V ranges from 1.9 to 7.4 nm; (3) the initial adhesion strength of fibroblast ranges from 58 to 143 nN, and it is negatively correlated to the Ra ; (4) the passivation results in distinguishable morphologies, which further substantiate the negative correlation between cell initial adhesion force and Ra ; and (5) our results fall short of confirming previous reports that found positively charged functional groups promoting fibroblast attachment and spread. Potential causes of the inconsistency are addressed. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res 2009