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Different regulation of hepatocyte behaviors between natural extracellular matrices and synthetic extracellular matrices by hepatocyte growth factor
Author(s) -
Hoshiba Takashi,
Wakejima Mai,
Cho ChongSu,
Shiota Goshi,
Akaike Toshihiro
Publication year - 2008
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31571
Subject(s) - hepatocyte growth factor , hepatocyte , extracellular matrix , fibronectin , laminin , extracellular , integrin , microbiology and biotechnology , growth factor , materials science , chemistry , biology , biochemistry , cell , receptor , in vitro
The roles of growth factors and extracellular matrices (ECMs) in regulation of hepatocyte behaviors are very important for the establishment of liver‐tissue engineering. Especially, collaboration between growth factors and ECMs is a big concern for liver‐tissue engineering. In this study, the hepatocyte responses by hepatocyte growth factor (HGF) were compared between natural ECMs and a synthetic galactose‐carrying polymer: poly( N ‐ p ‐vinylbenzyl‐4‐ O ‐β‐ D ‐galactopyranosyl‐ D ‐gluconamide) (PVLA). Hepatocytes underwent proliferation on type I collagen‐ and fibronectin‐coated surfaces in the presence of HGF, whereas hepatocytes formed spheroid on laminin‐1‐, PVLA‐, and poly‐ L ‐lysine (PLL)‐coated surfaces in the presence of HGF without the activation of proliferation. HGF accelerated ECM deposition, especially laminin‐10/11, beneath the hepatocytes cultured on PVLA‐ and PLL‐coated surfaces and the deposited laminin‐10/11 activated integrin signaling to collaborate with HGF signaling. Therefore, the deposited ECM molecules should be focused to clear the mechanism of hepatocyte behaviors in the presence of HGF. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res 2008