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Influence of nanohydroxyapatite patterns deposited by electrohydrodynamic spraying on osteoblast response
Author(s) -
Thian E. S.,
Huang J.,
Ahmad Z.,
Edirisinghe M. J.,
Jayasinghe S. N.,
Ireland D. C.,
Brooks R. A.,
Rushton N.,
Best S. M.,
Bonfield W.
Publication year - 2008
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31564
Subject(s) - materials science , vinculin , osteoblast , extracellular matrix , adhesion , nanoscopic scale , electrohydrodynamics , biophysics , cytoskeleton , cell adhesion , composite material , in vitro , microbiology and biotechnology , nanotechnology , cell , electrode , biology , chemistry , biochemistry
Electrohydrodynamic spraying has been used to produce patterns of line width up to 100 μm in size on glass discs, using nanohydroxyapatite (nHA). A human osteoblast (HOB)‐like cell model was then used to study the interaction between the HOB cells and nHA patterns in vitro . Growth of the cells was significantly increased ( p < 0.05) on the nHA surfaces. In addition, HOBs attached and spread well, secreting extracellular matrix. It was found that a confluent, aligned cell layer was achieved on nHA patterns by day 9. Immunofluorescent staining indicated that these cells showed elongated nuclei, enhanced adhesion (vinculin adhesion plaques) and a well‐aligned cytoskeleton (actin stress fibres). This work suggests that this type of spraying may provide a route for the production of nanoscale features on implants for biomedical applications. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res 2008