Influence of nanohydroxyapatite patterns deposited by electrohydrodynamic spraying on osteoblast response | Zendy

Thian E. S. | Zendy; Huang J. | Zendy; Ahmad Z. | Zendy; Edirisinghe M. J. | Zendy; Jayasinghe S. N. | Zendy; Ireland D. C. | Zendy; Brooks R. A. | Zendy; Rushton N. | Zendy; Best S. M. | Zendy; Bonfield W. | Zendy

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Influence of nanohydroxyapatite patterns deposited by electrohydrodynamic spraying on osteoblast response

Author(s) -

Thian E. S.,

Huang J.,

Ahmad Z.,

Edirisinghe M. J.,

Jayasinghe S. N.,

Ireland D. C.,

Brooks R. A.,

Rushton N.,

Best S. M.,

Bonfield W.

Publication year - 2008

Publication title -

journal of biomedical materials research part a

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.849

H-Index - 150

eISSN - 1552-4965

pISSN - 1549-3296

DOI - 10.1002/jbm.a.31564

Subject(s) - materials science , vinculin , osteoblast , extracellular matrix , adhesion , nanoscopic scale , electrohydrodynamics , biophysics , cytoskeleton , cell adhesion , composite material , in vitro , microbiology and biotechnology , nanotechnology , cell , electrode , biology , chemistry , biochemistry

Electrohydrodynamic spraying has been used to produce patterns of line width up to 100 μm in size on glass discs, using nanohydroxyapatite (nHA). A human osteoblast (HOB)‐like cell model was then used to study the interaction between the HOB cells and nHA patterns in vitro . Growth of the cells was significantly increased ( p < 0.05) on the nHA surfaces. In addition, HOBs attached and spread well, secreting extracellular matrix. It was found that a confluent, aligned cell layer was achieved on nHA patterns by day 9. Immunofluorescent staining indicated that these cells showed elongated nuclei, enhanced adhesion (vinculin adhesion plaques) and a well‐aligned cytoskeleton (actin stress fibres). This work suggests that this type of spraying may provide a route for the production of nanoscale features on implants for biomedical applications. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res 2008

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