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Electrospun‐modified nanofibrous scaffolds for the mineralization of osteoblast cells
Author(s) -
Venugopal J.,
Low Sharon,
Choon Aw Tar,
Kumar A. Bharath,
Ramakrishna S.
Publication year - 2008
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31538
Subject(s) - osteoblast , materials science , mineralization (soil science) , polycaprolactone , apatite , ultimate tensile strength , nanofiber , tissue engineering , biomedical engineering , electrospinning , composite material , chemistry , in vitro , mineralogy , biochemistry , polymer , organic chemistry , medicine , nitrogen
Abstract Biocompatible polycaprolactone (PCL) and hydroxyapatite (HA) were fabricated into nanofibrous scaffolds for the mineralization of osteoblasts in bone tissue engineering. PCL and PCL/HA nanofibrous surface were modified using oxygen plasma treatment and showing 0° contact angle for the adhesion and mineralization of osteoblast cells. The fiber diameter, pore size and porosity of nanofibrous scaffolds were estimated to be 220–625 nm, 3–20 μm, and 87–92% respectively. The ultimate tensile strength of PCL was about 3.37 MPa and PCL/HA was 1.07 MPa to withstand the long term culture of osteoblasts on nanofibrous scaffolds. Human fetal osteoblast cells (hFOB) were cultured on PCL and PCL/HA surface modified and unmodified nanofibrous scaffolds. The osteoblast proliferation rate was significantly ( p < 0.001) increased in surface‐modified nanofibrous scaffolds. FESEM showed normal phenotypic cell morphology and mineralization occurred in PCL/HA nanofibrous scaffolds, HA acting as a chelating agent for the mineralization of osteoblast to form bone like apatite for bone tissue engineering. EDX and Alizarin Red‐S staining indicated mineral Ca 2+ and phosphorous deposited on the surface of osteoblast cells. The mineralization was significantly increased in PCL/HA‐modified nanofibrous scaffolds and appeared as a mineral nodule synthesized by osteoblasts similar to apatite of the natural bone. The present study indicated that the PCL/HA surface‐modified nanofibrous scaffolds are potential for the mineralization of osteoblast for bone tissue engineering. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008