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Enhanced nerve regeneration through a bilayered chitosan tube: The effect of introduction of glycine spacer into the CYIGSR sequence
Author(s) -
Wang Wei,
Itoh Soichiro,
Matsuda Atsushi,
Aizawa Tomoyasu,
Demura Makoto,
Ichinose Shizuko,
Shinomiya Kenichi,
Tanaka Junzo
Publication year - 2007
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31522
Subject(s) - chitosan , materials science , microfiber , electrospinning , regeneration (biology) , sciatic nerve , nanofiber , laminin , polyamide , glycine , biophysics , biomedical engineering , polymer , composite material , amino acid , anatomy , chemical engineering , extracellular matrix , biochemistry , microbiology and biotechnology , chemistry , medicine , biology , engineering
We have developed a novel bilayered chitosan tube that comprises an outer layer of chitosan film and an inner layer of chitosan nonwoven nano/microfiber mesh. The tube is fabricated with an electrospinning method. We characterized the microstructure and mechanical properties of this material. We introduced glycine spacers into the C YIGSR sequence, domain of laminin‐1 that enhances Schwann cells migration and attachment, as well as neural outgrowth, resulting in the amino acid sequences CGG YIGSR and C YIGSR . These peptides were covalently bound to the nano/microfiber mesh surface of the chitosan tube to examine the effects of peptide mobility on nerve regeneration. Scaffolds constructed from these bilayered chitosan tubes were grafted to bridge injured sciatic nerve. Isografting was performed as a control. These scaffolds were removed 5 and 10 weeks after implantation for histological analysis. Nerve regeneration into chitosan tubes, on which the C YIGSR peptide was immobilized, exhibited efficacy similar to that of the isograft and represent a promising candidate for promoting peripheral nerve repair. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008