Premium
N ‐halamine‐based chitosan: Preparation, characterization, and antimicrobial function
Author(s) -
Cao Zhengbing,
Sun Yuyu
Publication year - 2008
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31463
Subject(s) - chitosan , bleach , antimicrobial , materials science , chlorine , bacteria , nuclear chemistry , x ray photoelectron spectroscopy , titration , biofilm , microbiology and biotechnology , organic chemistry , chemistry , chemical engineering , biology , genetics , metallurgy , engineering
Upon chlorine bleach treatment, amino groups in chitosan were transformed into N ‐halamine structures. The transformation was confirmed by UV/VIS, XPS, DSC, and TGA evaluation and iodimetric titration. The N ‐halalmine‐based chitosan provided total kill of 10 8 –10 9 colony forming units (CFU/mL) of E. coli (gram‐negative bacteria) and S. aureus (gram‐positive bacteria) in 10 and 60 min, respectively. SEM observations demonstrated that the chlorinated chitosan effectively prevented the formation of bacterial biofilms. The antimicrobial activity and bio film controlling function were stable for longer than 1 month; when the functions were lost due to extensive use and/or prolonged storage, they could be readily recharged by another bleach treatment. The antimicrobial mechanism was also discussed. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res 2008