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Regional variability, processing methods, and biophysical properties of human fascia lata extracellular matrix
Author(s) -
Derwin Kathleen A.,
Baker Andrew R.,
Spragg Rebecca K.,
Leigh Diane R.,
Farhat Walid,
Iannotti Joseph P.
Publication year - 2008
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31455
Subject(s) - fascia lata , hydroxyproline , materials science , extracellular matrix , biomedical engineering , anatomy , fascia , tendon , medicine , chemistry , biochemistry
This study aims to assess the regional variability, processing methods, mechanical, biochemical, and cellular properties of human fascia lata as a scaffold for soft tissue repair and tissue engineering applications. Ten pairs of fascia lata (donor age 18–55) were used. One fascia patch from each pair was used to assess the geometric and biomechanical variability of fresh fascia. The other from each pair was subjected to 1 of 2 allograft processing methods: antibiotic soak alone or acellularization plus antibiotic soak. Stiffness, modulus, hydroxyproline, chondroitin/dermatan sulfate glycosaminoglycan (CSDS GAG), and DNA content were quantified in fascia from fresh and treated groups. The effect of location was not significant for thickness or stiffness within a 6 × 12 cm 2 region of the iliotibial tract of fresh human fascia lata. Processing did not significantly change the stiffness, modulus, or CSDS GAG content of fascia ECM. However, hydroxyproline (collagen) content is significantly reduced in acellularized fascia, probably reflecting a removal of soluble collagen during the treatment ( p < 0.02). Processing reduced the DNA content of fresh fascia approximately 10‐fold ( p < 0.001). The mechanical, chemical and ultrastructural similarities between fascia lata and tendon may make fresh or processed fascia an attractive ECM scaffold for soft tissue, particularly tendon, repair. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008