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Spatial distribution of mineralized bone matrix produced by marrow mesenchymal stem cells in self‐assembling peptide hydrogel scaffold
Author(s) -
Hamada Kazunori,
Hirose Motohiro,
Yamashita Toshihiko,
Ohgushi Hajime
Publication year - 2008
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31439
Subject(s) - mesenchymal stem cell , extracellular matrix , materials science , self healing hydrogels , osteocalcin , microbiology and biotechnology , scaffold , alkaline phosphatase , biomedical engineering , tissue engineering , ascorbic acid , matrix (chemical analysis) , biomaterial , biology , biochemistry , nanotechnology , medicine , composite material , food science , polymer chemistry , enzyme
Abstract We evaluated the osteogenic differentiation of mesenchymal stem cells (MSCs) using a new class of synthetic self‐assembling peptide hydrogels, RADA 16, as a scaffold for three‐dimensional culture. MSCs derived from rat bone marrow were culture‐expanded and seeded into the hydrogel and further cultured in osteogenic medium containing β‐glycerophosphate, ascorbic acid, and dexamethasone for 2–4 weeks. High alkaline phosphatase activity and osteocalcin (OC) contents were detected at both the protein and gene expression levels during the culture periods. Both calcium and the OC contents increased over time, indicating the growth of a mineralized extracellular matrix within the hydrogel. Moreover, the process of the growth of the mineralized matrix determined by three‐dimensional microarchitecture images was obtained by confocal laser scanning microscopy. The findings show that MSCs can differentiate into mature osteoblasts to form mineralized matrices within the hydrogel scaffold. Importantly, the differentiation can occur three dimensionally within the hydrogel, indicating that RADA 16 can be considered attractive synthetic biomaterial for use in bone tissue engineering. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008