Premium
Modification of Ti implant surface for cell proliferation and cell alignment
Author(s) -
Zhao Jia Ming,
Tsuru Kanji,
Hayakawa Satoshi,
Osaka Akiyoshi
Publication year - 2007
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31426
Subject(s) - materials science , anatase , biocompatibility , titanium , coating , simulated body fluid , osteoblast , implant , surface modification , nanotechnology , biomedical engineering , chemical engineering , composite material , in vitro , biochemistry , chemistry , scanning electron microscope , metallurgy , photocatalysis , engineering , medicine , surgery , catalysis
Surface properties of implants are the keys for ensuring their long‐lasting anchorage to the tissue. This study aims to develop a novel implant surface microstructure with high biocompatibility and ability of guided tissue formation. By a photolithography method, gold (Au) grids (1 × 1 mm 2 square lattices, 10 μm in grid‐line width) were deposited on titanium substrates. They were oxidized with H 2 O 2 solution to yield titania (anatase) layer, and the Au grid formed channels due to larger molar volume of anatase than Ti. L ‐Cysteine and type I collagen were then immobilized on them to yield the target substrates, CHT–Au–cys–col. Apatite deposited within 3 days when they were soaked in Kokubo's simulated body fluid, regardless of the protein coating, but not on the bottom of the Au channel. Osteoblast‐like MC3T3‐E1 cells were cultured on the CHT–Au–cys–col substrates, showing that (1) the cysteine–collagen coating promoted cell attachment and proliferation, and (2) the Au channels were filled with the cells which were aligned along the channel direction and were connected to the neighboring cells as well as attached to the channel wall with cytoplasmic extensions. The results thus ensured filopodial guidance for the substrates. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008