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A study on polysialic acid as a biomaterial for cell culture applications
Author(s) -
Stark Yvonne,
Bruns Stephanie,
Stahl Frank,
Kasper Cornelia,
Wesemann Maike,
Grothe Claudia,
Scheper Thomas
Publication year - 2008
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31406
Subject(s) - polysialic acid , hyaluronic acid , cell culture , materials science , cell adhesion , adhesion , neural cell adhesion molecule , coating , viability assay , biomaterial , tissue engineering , cell , scaffold , mtt assay , biomedical engineering , biochemistry , biophysics , chemistry , nanotechnology , biology , anatomy , composite material , medicine , genetics
Polysialic acid (PSA) was investigated for its applicability as coating material for mammalian cell cultivation. PSA is involved in post‐translational modification of the vertebrate neural cell adhesion molecule (NCAM). It is biocompatible and degradation‐controlled. Thus, it becomes interesting for use as a coating and scaffold material for tissue engineering applications, especially for peripheral nerve regeneration. As a preliminary study of the use of PSA as scaffold material it was tested in its soluble form as coating material. The cytotoxicity was investigated and compared to another polysaccharide β‐glucan, to widely used coating substances (collagen I, poly‐ L ‐lysine, hyaluronic acid) and uncoated tissue culture plastic material. The interactions between the modified cell culture surface and the cells were investigated using a model liver cell line Hep‐G2 and a neurobiological cell line PC‐12. The PSA coating itself was analyzed by immunoanalysis. Viability of the cells was investigated by the MTT assay. The number and distribution of adhered cells were studied by cell nuclei staining. Furthermore, the differentiation status of the PC‐12 cells was monitored, as well as glucose and lactate levels in the cell culture medium from the Hep‐G2 cells. Comparable viability and similar numbers of attached cells were observed. Growth in cell clusters was observed for PSA, β‐glucan, and hyaluronic acid coated materials. In general, the results indicate that PSA is comparable to other well‐established coating materials (e.g. collagen I, hyaluronic acid, and poly‐ L ‐lysine). Moreover, as a key substance in vertebrate development it offers interesting features for nerve regeneration, especially as an insoluble, modified scaffold material. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008