Premium
Antibiotic multiresistance strictly associated with IS 256 and ica genes in Staphylococcus epidermidis strains from implant orthopedic infections
Author(s) -
Montanaro Lucio,
Campoccia Davide,
Pirini Valter,
Ravaioli Stefano,
Otto Michael,
Arciola Carla Renata
Publication year - 2007
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31399
Subject(s) - staphylococcus epidermidis , gentamicin , clindamycin , microbiology and biotechnology , vancomycin , penicillin , antibiotics , ciprofloxacin , biology , antibiotic resistance , staphylococcus aureus , genetics , bacteria
In this study the presence both of the ica genes, encoding for biofilm exopolysaccharide production, and the insertion sequence IS 256 , a mobile element frequently associated to transposons, was investigated in relationship with the prevalence of antibiotic resistance among Staphylococcus epidermidis strains. The investigation was conducted on 70 clinical isolates derived from orthopedic implant infections. Among the clinical isolates investigated a dramatic high level of association was found between the presence of ica genes as well as of IS 256 and multiple‐resistance to all the antibiotics tested (oxacillin, penicillin, gentamicin, erythromycin, clindamycin, chloramphenicol, sulfamethoxazole + trimethoprim, ciprofloxacin, vancomycin). Noteworthy, a striking full association between the presence of IS 256 and resistance to gentamicin was found, being none of the IS 256 ‐negative strain resistant to this antibiotic. This association is probably because of the link of the corresponding aminoglycoside‐resistance genes, and IS 256 , often co‐existing within the same staphylococcal transposon. In conclusion, in orthopedics, the presence of ica genes and that of IS 256 in S. epidermidis genome should both be considered as informative markers of clinically relevant strains equipped with greatest and broadest resistance potential to survive to medical treatments. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007