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Proliferative capacity and osteogenic potential of novel dura mater stem cells on poly‐lactic‐ co ‐glycolic acid
Author(s) -
Petrie Caren,
Tholpady Sunil,
Ogle Roy,
Botchwey Edward
Publication year - 2008
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31367
Subject(s) - plga , dura mater , stem cell , glycolic acid , tissue engineering , biomedical engineering , regeneration (biology) , scaffold , microbiology and biotechnology , extracellular matrix , materials science , chemistry , in vitro , anatomy , lactic acid , medicine , biology , biochemistry , genetics , bacteria
The rational design of biomimetic structures for the regeneration of damaged or missing tissue is a fundamental principle of tissue engineering. Multiple variables must be optimized, ranging from the scaffold type to the selection and properties of implanted cell(s). In this study, the osteogenic potential of a novel stem cell was analyzed on biodegradable poly(lactic‐ co ‐glycolic acid) (PLGA) biomaterials as a step toward creating new cell‐materials constructs for bony regeneration. Dura mater stem cells (DSCs), isolated from rat dura mater, were evaluated and compared to bone marrow stem cells (BMSCs) for proliferative and differentiative properties in vitro . Experiments were carried out on both tissue culture plastic (TCP) and 2D planar films of PLGA. Proliferation of DSCs on both TCP and PLGA films increased over 21 days. Positive fold inductions in all five bone marker genes were observed at days 7, 14, 21 in all experimental samples compared with day 0 controls. DSCs demonstrated greater cell coverage and enhanced matrix staining on 2D PLGA films when compared with BMSCs. These cells can be isolated and expanded in culture and can subsequently attach, proliferate, and differentiate on both TCP and PLGA films to a greater extent than BMSCs. This suggests that DSCs are promising for cell‐based bone tissue engineering therapies, particularly those applications involving regeneration of cranial bones. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res 2008