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Gastric cancer cells in collagen gel matrix: Three‐dimensional growth and differential expression of adhesion molecules (CD44s, CD54, E‐cadherin)
Author(s) -
Jia Qian,
Feng Meifu,
Wang Yun,
Xue Shaobai
Publication year - 2007
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31352
Subject(s) - cancer cell , flow cytometry , cell adhesion , matrix (chemical analysis) , adhesion , cell culture , cell adhesion molecule , cell growth , materials science , cell , cadherin , cancer , monolayer , microbiology and biotechnology , biology , biochemistry , nanotechnology , genetics , composite material
To characterize the growth of human gastric cancer cells in collagen gel matrix and adhesive status of the cells in comparison with conventional monolayer cells. Three kinds of human gastric cancer cell lines (BGC823, SGC7901, and MKN28) were cultured alone or co‐cultured with normal human fibroblasts in collagen gel matrix, and their cell cycle, metabolic function, and the expression of adhesive molecules (CD44s, CD54, and E‐cadherin) were analyzed by flow cytometry or other methods. Two of three cell lines (BGC823 and SGC7901) and their co‐cultures showed multilayer growth in collagen gel matrix, and their growth and metabolism rate became slow and the cell adhesion molecules (CAMs) expression was down regulated. Gastric cancer cell alone or with fibroblasts in collagen gel matrix showed distinct growth feature when compared with monolayer cells, which represent two kinds of different experimental models. BGC823 and SGC7901 cells growing in three‐dimension may recur some characteristics of their original solid tumor in vivo with the invasive or metastatic ability. According to different aims, it should pay great care in choice of experimental model to get more reasonable results. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008