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Acute oral toxicity evaluation of biodegradable and pH‐sensitive hydrogel based on polycaprolactone, poly(ethylene glycol) and methylacrylic acid (MAA)
Author(s) -
Chen Xian,
Qian ZhiYong,
Gou MaLing,
Chao GuoTao,
Zhang YangDe,
Gu YingChun,
Huang MeiJuan,
Wang JiWei,
Pan YiFeng,
Wei YuQuan,
Chen JianPing,
Tu MingJing
Publication year - 2007
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31350
Subject(s) - ethylene glycol , acute toxicity , toxicity , polycaprolactone , self healing hydrogels , materials science , pharmacology , oral administration , median lethal dose , spleen , nuclear chemistry , h&e stain , chemistry , polymer chemistry , medicine , staining , pathology , organic chemistry , composite material , polymer
In this article, a novel biodegradable and pH‐sensitive hydrogel based on polycaprolactone, poly(ethylene glycol) and methylacrylic acid (MAA), was prepared by UV‐initiated free radical polymerization. The obtained hydrogel was characterized by 1 H NMR and FTIR. The acute toxicity tests and histopathological study were performed in BALB/c mice. In acute oral toxicity test, mice were orally administered with a total 15 g/kg body weight (b.w.) of P(CL‐MAA‐EG) hydrogels, and were observed continuously for 14 days. For histopathologic study, samples including heart, liver, lung, kidneys, spleen, stomach, and intestine, were histochemically prepared and stained with hematoxylin‐eosin for histopathologic examination. No mortality or significant signs of acute toxicity was observed during the whole observation period, and no macroscopic alteration was found in the organs. Histopathological analysis of various organs also did not show any significant pathological changes. Thus, the maximal tolerance dose of P(CL‐MAA‐EG) hydrogels was calculated to be higher than 15 g/kg b.w. in BALB/c mice. It was suggested that the studied P(CL‐MAA‐EG) hydrogel in this article were nontoxic after acute oral administration and it might be a promising candidate as a novel oral drug carrier. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008