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Silklike materials constructed from sequences of Bombyx mori silk fibroin, fibronectin, and elastin
Author(s) -
Yang Mingying,
Tanaka Chikako,
Yamauchi Kazuo,
Ohgo Kosuke,
Kurokawa Masato,
Asakura Tetsuo
Publication year - 2008
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31348
Subject(s) - fibroin , bombyx mori , materials science , silk , elastin , sericin , polymer science , fibronectin , composite material , biology , biochemistry , genetics , extracellular matrix , gene
Two silklike proteins, [ TGRGDSPAGG(GAGAGS) 3 AS ] 5 ( FS 5 ) and [ TGRGDSPA‐(GVPGV) 2 GG(GAGAGS) 3 AS ] 8 ( FES 8 ) were designed to demonstrate the superior performance as biomaterials of silklike proteins. The former protein consists of the crystalline domain sequence, ( GAGAGS )n from Bombyx mori silk fibroin and cell‐adhesive sequence TGRGDSPA coming from fibronectin‐containing RGD triplet. The additional sequence ( GVPGV ) n from elastin was included in the latter protein. The considerably higher cell‐adhesion activities of these proteins for NHDF and VERO cells were observed by comparing with those of silklike materials without RGD sequences and also the crystalline fraction of B. mori silk fibroin. This tendency was independent of the treatments, 4.5 M LiClO 4 or formic acid (FA), on silklike proteins. Their activities are also higher than those of commercial Fibronectin F™ for NHDF cell. Their structural characterization was studied using 13 C solid‐state NMR. Although the overlapped peaks in usual 13 C CP/MAS NMR spectra make the detailed structural analysis difficult, the methyl resonance regions observed using dipolar dephasing NMR were very useful for the analysis. The presence of both random coil and β‐sheet structures was observed in these proteins clearly. The content of β‐sheet structure in both proteins increases after FA treatment when compared with the lyophilized samples. The production of electrospun nanofibers from their hexafluoroacetone solution was also tried. The silklike protein FES 8 could prepare nonwoven silk fibers although FS 5 could not. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008