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Bone cell responses to the composite of Ricinus communis polyurethane and alkaline phosphatase
Author(s) -
Beloti Marcio Mateus,
de Oliveira Paulo Tambasco,
Tagliani Marcela Martini,
Rosa Adalberto Luiz
Publication year - 2008
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31344
Subject(s) - alkaline phosphatase , simulated body fluid , incubation , viability assay , materials science , biomaterial , chemistry , biochemistry , andrology , nuclear chemistry , microbiology and biotechnology , biology , in vitro , medicine , mineralogy , enzyme , nanotechnology , apatite
The aim of this study was to evaluate the response of osteoblastic cells to the composite of Ricinus communis polyurethane (RCP) and alkaline phosphatase (ALP) incubated in synthetic body fluid (SBF). RCP pure (RCPp) and RCP blended with ALP 6 mg/mL polymer (RCP+ALP) were incubated in SBF for 17 days. Four groups of RCP were tested: RCPp, RCP+ALP, and RCPp and RCP+ALP incubated in SBF (RCPp/SBF and RCP+ALP/SBF). Stem cells from rat bone marrow were cultured in conditions that allowed osteoblastic differentiation on RCP discs and were evaluated: cell adhesion, culture growth, cell viability, total protein content, ALP activity, and bone‐like nodule formation. Data were compared by ANOVA or Kruskal–Wallis test. The group RCP+ALP was highly cytotoxic and, therefore, was not considered here. Cell adhesion ( p = 0.14), culture growth ( p = 0.39), viability ( p = 0.46) and total protein content ( p = 0.12) were not affected by either RCP composition or incubation in SBF. ALP activity was affected ( p = 0.0001) as follows: RCPp < RCPp/SBF < RCP+ALP/SBF. Bone‐like nodule formation was not observed on all evaluated groups. The composite RCP+ALP prior to SBF incubation is cytotoxic and must not be considered as biomaterial, but the incorporation of ALP to the RCP followed by SBF incubation could be a useful alternative to improve the biological properties of the RCP. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008