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Enhancement of cellular binding efficiency and cytotoxicity using polyethylene glycol base triblock copolymeric nanoparticles for targeted drug delivery
Author(s) -
Yang Jaemoon,
Cho EunJin,
Seo Sungbaek,
Lee JaeWon,
Yoon HoGeun,
Suh JinSuck,
Huh YongMin,
Haam Seungjoo
Publication year - 2008
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31312
Subject(s) - polyethylene glycol , materials science , nanoparticle , drug delivery , cytotoxicity , dynamic light scattering , copolymer , drug carrier , ethylene glycol , peg ratio , conjugated system , bioconjugation , nuclear chemistry , chemical engineering , biophysics , polymer , nanotechnology , organic chemistry , chemistry , in vitro , biochemistry , finance , economics , composite material , engineering , biology
Folate (FA) conjugated tri‐block copolymers were prepared by bioconjugation of poly ε‐caprolactonediol and various molecular weights of diamine polyethylene glycol. The synthetic tri‐block copolymers were characterized by 1 H‐NMR. Three types of nanoparticles were prepared by nanoprecipitation. Their size and morphology were verified by laser scattering and transmission electron microscopy, respectively. The colloidal stability of the nanoparticles was evaluated by turbidity test. The anticancer drug doxorubicin (DOX) was encapsulated in the nanoparticles during preparation. Drug loading amounts and release behavior from prepared nanoparticles were investigated. Fluorescent‐activated cell sorting analysis and epi‐fluorescencic microscopic imaging of prepared nanoparticles exhibited good cellular uptake against target cells. FA receptor expressed OVCAR3 cells that showed higher mean fluorescence intensity than FA receptor defect A549 cells at specific polyethylene glycol chain lengths. The cell cytotoxicity of prepared nanoparticles was evaluated for receptor mediated drug delivery. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008