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Quantitative analysis of cell adhesion on aligned micro‐ and nanofibers
Author(s) -
Tian Furong,
Hosseinkhani Hossein,
Hosseinkhani Mohsen,
Khademhosseini Ali,
Yokoyama Yoshiro,
Estrada Giovani Gomez,
Kobayashi Hisatoshi
Publication year - 2008
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31304
Subject(s) - materials science , nanofiber , adhesion , scanning electron microscope , composite material , composite number , cell adhesion , tissue engineering , morphology (biology) , electrospinning , mixing (physics) , biomedical engineering , polymer , medicine , physics , quantum mechanics , biology , genetics
In this study, we quantitatively analyzed the affinity of cell adhesion to aligned nanofibers composed of composites of poly(glycolic acid) (PGA) and collagen. Electrospun composite fibers were fabricated at various PGA/collagen weight mixing ratio (7, 18, 40, 67, and 86%) to generate fibers that ranged in diameter from 10 μm to 500 nm. Scanning electron microscopy (SEM) observation revealed that the PGA/collagen fibers were long and uniformly aligned, irrespective of the PGA/collagen weight mixing ratio. In addition, it was observed that a significantly higher number of NIH3T3 fibroblasts adhered to nanofibers with smaller diameters in comparison to fibers with larger diameters. The highest affinity of cell adhesion was observed in the PGA/collagen fibers with diameter of 500 nm and PGA/collagen weight mixing ratio of 40%. Furthermore, the adherent cells were more elongated on fibers with smaller diameters. Thus, based on the results here, PGA/collagen composite fibers are suitable for tissue culture studies and provide an attractive material for tissue engineering applications. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008