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Enhanced osteoblast‐like cell adhesion and proliferation using sulfonate‐bearing polymeric scaffolds
Author(s) -
Chaterji Somali,
Gemeinhart Richard A.
Publication year - 2007
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31283
Subject(s) - self healing hydrogels , extracellular matrix , adhesion , materials science , cell adhesion , osteoblast , monomer , biophysics , tissue engineering , in vitro , biomedical engineering , microbiology and biotechnology , polymer chemistry , chemistry , polymer , biochemistry , biology , composite material , medicine
Orthopedic malfunction, degeneration, or damage remains a serious healthcare issue despite advances in medical technology. Proactive extracellular matrix (ECM)‐mimetic scaffolds are being researched to orchestrate the activation of diverse osteogenic signaling cascades, facilitating osteointegration. We hypothesized that sulfonated functionalities incorporated into synthetic hydrogels would simulate anionic, sulfate‐bearing proteoglycans, abundant in the ECM. Using this rationale, we successfully developed differentially sulfonated hydrogels, polymerizing a range of sulfopropyl acrylate potassium‐acrylamide (SPAK‐AM) mole ratios as monomer feeds under room temperature conditions. For anchorage‐dependent cells, such as osteoblasts, adhesion is a critical prerequisite for subsequent osteointegration and cell specialization. The introduction of the sulfonated monomer, SPAK, resulted in favorable uptake of serum proteins with proportional increase in adhesion and proliferation rates of model cell lines, which included NIH/3T3 fibroblasts, MG‐63 osteoblasts, and MC3T3‐E1 subclone 4 preosteoblasts. In fact, higher proportions of sulfonate content (pSPAK75, pSPAK100) exhibited comparable or even higher degrees of adhesion and proliferation, relative to commercial grade tissue culture polystyrene in vitro . These results indicate promising potentials of sulfonated ECM‐mimetic hydrogels as potential osteogenic tissue engineering scaffolds. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007