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Development of mature cartilage constructs using novel three‐dimensional porous scaffolds for enhanced repair of osteochondral defects
Author(s) -
Kasahara Yasuhiko,
Iwasaki Norimasa,
Yamane Shintaro,
Igarashi Tatsuya,
Majima Tokifumi,
aka Sachiko,
Harada Kazuo,
Nishimura ShinIchiro,
Minami Akio
Publication year - 2008
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31259
Subject(s) - materials science , cartilage , biomedical engineering , hyaline cartilage , tissue engineering , scaffold , hyaluronic acid , chondrogenesis , regeneration (biology) , anatomy , articular cartilage , osteoarthritis , medicine , pathology , biology , microbiology and biotechnology , alternative medicine
Abstract In this study, we successfully developed two types of volume‐reduced three‐dimensional scaffolds, including cushion‐ and cylinder‐shape scaffolds, fabricated from chitosan‐based hyaluronic acid hybrid polymer fibers. Using these scaffolds combined with a bioreactor system, we regenerated histologically and mechanically mature cartilage constructs. The final goal of this study was to clarify the ability of this engineered cartilage construct to induce cartilage repair in osteochondral defects. The mature cartilage constructs regenerated with two types of scaffolds were implanted into 5‐mm diameter osteochondral defects in the patellar groove of rabbits. At 12 weeks after implantation, the reparative tissues consisted of hyaline‐like cartilage with evidence of stable fusion to adjacent native cartilage and normal reconstitution of subchondral bone. The histological score of these tissues significantly outranked the value of untreated tissue. Biomechanically, compression modulus of reparative tissue at 12 weeks postoperatively was comparative to that of normal articular cartilage. Our results indicate that the implantation of constructs with mature cartilage have potential as a better approach for joint resurfacing. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008