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Influence of the surface structure of a multiblock copolymer on the cellular behavior of primary cell cultures of the upper aerodigestive tract in vitro
Author(s) -
Rickert Dorothee,
Franke R.P.,
Lendlein A.,
Kelch S.,
Moses M.A.
Publication year - 2007
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31250
Subject(s) - extracellular matrix , materials science , adhesion , cell adhesion , matrix metalloproteinase , biomaterial , in vitro , biophysics , cell growth , zymography , copolymer , cell , kinetics , biomedical engineering , microbiology and biotechnology , nanotechnology , chemistry , biochemistry , medicine , biology , polymer , composite material , physics , quantum mechanics
The influence of the surface topography of a biodegradable copolymer on adhesion, proliferation, and cellular activity of primary cell cultures of the upper aerodigestive tract (ADT) was investigated. On the basis of the important functions of matrix metalloproteinases (MMPs) and their endogenous inhibitors, tissue inhibitor of MMPs (TIMPs) in regulating extracellular matrix remodeling, cellular adhesion and growth, the appearance and kinetics of these enzymes were investigated in primary cells of the upper ADT seeded on different surfaces of a polymeric biomaterial. Primary cell cultures of the upper ADT of Sprague‐Dawley rats were seeded on different surfaces (smooth versus rough surface) of a biodegradable multiblock copolymer and on polystyrene surface as control. Conditioned media of the primary cells were analyzed for MMPs and TIMPs by both zymography and radiometric enzyme assay. Cell adhesion and proliferation as well as the kinetics of appearance and activity level of MMP‐1, MMP‐2, and TIMPs were significantly different depending on the cell type and the surface structure of the multiblock copolymer. In this study, the data obtained indicated that surface topography governed the biological response to biomaterials. Knowledge as to how cells interact with the interface of biomaterials will be necessary in order to eventually design the “ideal” surface of biomaterials, which will be both tissue and organ‐optimized in order to best provide clinicians with specific and viable novel therapeutical options in medicine. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007