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In vitro and in vivo biological responses of plasma‐sprayed hydroxyapatite coatings with posthydrothermal treatment
Author(s) -
Yang C. Y.,
Lee T. M.,
Yang C. W.,
Chen L. R.,
Wu M. C.,
Lui T. S.
Publication year - 2007
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31246
Subject(s) - materials science , crystallinity , in vivo , implant , apposition , biomedical engineering , osseointegration , composite material , anatomy , surgery , biology , medicine , microbiology and biotechnology
This study was undertaken to evaluate the effect of post‐hydrothermal treatment on the biological responses of the plasma‐sprayed hydroxyapatite (HA)‐coated Ti‐6Al‐4V implant system both in vitro and in vivo . After hydrothermal treatment, the HA coating (HAC) shows the high mechanical strength and indices‐of‐crystallinity, denser microstructure, lower concentrations of amorphous and impurity phases, when compared with the as‐sprayed HAC. The in vitro cell‐culture studies, using UMR106 osteoblast‐like cell, demonstrated no signifiacnt cell growth on both surface of as‐sprayed and hydrothermal‐treated HACs during 10‐day culture. The in vivo studies, using the transcortical implant model in the femora of goats, evaluated the histological responses of two coatings. After 6 week of implantation, using backscattered electron images, no substantial histological variations in the extents of new bone apposition and new bone healing between the two HACs were observed. However, the as‐sprayed HAC, owing to the dissolution induced the granular particles dissociated from the HAC, showed the statically lower extent of new bone apposition than hydrothermal‐treated HAC at 12 weeks. The results suggest that hydrothermal treatment could be used to improve the mechanical strength, crystallinity, and phase composition of HAC, which are important factors of long‐term fixation and stability of implant. Besides, the treated HAC could also achieve the initial fixation of implant in clinical use. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res 2007