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Soft tissue reactions to bioactive glass 13‐93 combined with chitosan
Author(s) -
Asikainen A.J.,
Hagström J.,
Sorsa T.,
Noponen J.,
Kellomäki M.,
Juuti H.,
Lindqvist C.,
Hietanen J.,
Suuronen R.
Publication year - 2007
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31225
Subject(s) - bioactive glass , chitosan , immunohistochemistry , eosinophilic , materials science , matrix (chemical analysis) , matrix metalloproteinase , subcutaneous injection , pathology , allergic reaction , subcutaneous tissue , chemistry , immunology , medicine , allergy , biochemistry , composite material
The aim of this study was to evaluate rabbit soft tissue reactions to bioactive glass 13‐93 mesh by using a histological and immunohistochemical analysis. Bioactive glass (13‐93) mesh fixed with 3 wt % chitosan was implanted into the dorsal subcutaneous space of New Zealand White rabbits ( n = 18) for six, 12, and 24 weeks, respectively. After 6 weeks the bioactive glass remnants were surrounded by foreign‐body granuloma with eosinophilic granulocytes. After 12 and 24 weeks the implanted material was mainly absorbed, but, if any particles still remained the foreign‐body reaction was notably milder. Yet, a mild chronic inflammatory infiltrate was present. Matrix metalloproteinase (MMP) ‐2, ‐3, ‐13 and tissue inhibitory protein (TIMP‐1 and ‐2) expressions were studied by immunohistochemistry. MMP‐3, ‐13, TIMP‐1, and ‐2 positivity were detected throughout the follow‐up period. MMP‐2 positivity was only occasionally seen in the 24 week samples, which is constitutively expressed but is not related to inductive MMP‐3 and ‐13 cascade. The presence of eosinophilic granulocytes in some of the samples raises the possibility of an allergic reaction to the materials. MMP‐3 and ‐13 are suggested to participate in the host reaction to either bioactive glass or chitosan. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007