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Fabrication of nanostructured hydroxyapatite and analysis of human osteoblastic cellular response
Author(s) -
Guo Xingyuan,
Gough Julie E.,
Xiao Ping,
Liu Jing,
Shen Zhijian
Publication year - 2007
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31200
Subject(s) - materials science , sintering , spark plasma sintering , particle size , ceramic , mineralization (soil science) , equiaxed crystals , scanning electron microscope , chemical engineering , composite material , microstructure , chemistry , organic chemistry , nitrogen , engineering
Nano‐sized hydroxyapatite (HA) powders were produced by a hydrothermal method and a precipitation method. Spark plasma sintering (SPS) was used to fabricate nanostructured HA (NHA) using nano‐sized HA powders as a precursor. Conventional sintering was employed to produce microstructured HA (MHA). Characteristics of HA powders and HA bulk ceramics after sintering were investigated by XRD, FTIR, SEM, TEM, particle size distribution, and AFM. Dense compacts consisting of equiaxed grains with an average grain size of ∼100 nm were obtained by SPS. Human osteoblasts were cultured on both NHA and MHA and cell attachment, proliferation, and mineralization were evaluated. After 90 min incubation, the cell density on NHA surface was significantly higher than that of MHA and glass control, whereas average cell area of a spread cell was significantly lower on NHA surface compared to MHA and glass control after 4 h incubation. Matrix mineralization was determined after 7 and 14 days incubation by using alizarin red assay combined with cetylpyridinium chloride extraction. NHA shows significant enhancement ( p < 0.05) in mineralization compared to MHA. Results from this study suggest that NHA may be a much better candidate for clinical use in terms of bioactivity. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007