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Immobilization of homing peptide on magnetite nanoparticles and its specificity in vitro
Author(s) -
Gan ZhiFeng,
Jiang JiSen,
Yang Yong,
Du Bing,
Qian Min,
Zhang Ping
Publication year - 2008
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31181
Subject(s) - homing (biology) , materials science , peptide , green fluorescent protein , magnetic nanoparticles , fluorescence microscope , in vitro , nanoparticle , magnetic hyperthermia , biophysics , magnetite nanoparticles , fluorescence , microbiology and biotechnology , biochemistry , nanotechnology , chemistry , biology , optics , ecology , physics , gene
As a homing peptide, A54 is the most effective peptide specific to the human hepatocellular carcinoma cell. Homing peptide labeled with green fluorescent protein (A54‐GFP) was successfully immobilized on the surfaces of magnetic nanoparticles and characterized by Fourier transform infrared spectroscopy as well as fluorescence microscopy. The binding efficiency was analyzed by performing adsorption equilibrium and SDS‐PAGE electrophoresis. Specific binding of the nanoparticles functionalized with A54‐GFP to human hepatocellular carcinoma cells in vitro was visualized using fluorescence microscopy. The results demonstrated the specificity of A54‐GFP‐coated magnetic nanoparticle to tumor cell, pointing to its great potential in magnetic cell separation and purification, magnetic resonance imaging (MRI), magnetic hyperthermia, and drug targeting. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008