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Effects of mannosylated glycopolymers on specific interaction to bone marrow hematopoietic and progenitor cells derived from murine species
Author(s) -
Park KeunHong,
Na Kun,
Lee Yong Su,
Chang WonKyong,
Park JinKi,
Akaike Thoshihiro,
Kim Dong Ku
Publication year - 2007
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31137
Subject(s) - haematopoiesis , flow cytometry , progenitor cell , microbiology and biotechnology , fluorescein isothiocyanate , fluorescence microscope , bone marrow , stem cell , monocyte , materials science , biology , fluorescence , immunology , physics , quantum mechanics
Poly[ N‐ pvinylbenzyl‐ O ‐ D ‐galactopyranosyl‐(1‐4)‐ D ‐glucoamide], poly[ N ‐pvinylbenzyl‐ O ‐ D ‐glucopyranosyl‐(1‐4)‐ D ‐glucoamide], and poly[ N ‐p‐vinylbenzyl‐ O ‐mannopyranosyl‐(1‐4)‐ D ‐gluconamide] (referred to as PVLA, PVMA, and PV‐Man) are polystyrene derivatives that contain galactose, glucose, and mannose moieties, which interact with hematopoietic cells (HCs). To clarify the specific interaction between the glucopolymers and hematopoietic cells, glycopolymers labeled with fluorescent isothiocyanate (FITC) were used to follow the specific interaction, which was visualized by confocal laser microscopy. We found that PV‐Man binds strongly to HCs, probably because of a specific interaction mediated by specific receptors present on the cell membrane, while some cytotoxicity when was observed when PV‐Man interacted with the cell membrane. The fluorescence intensity between PV‐Man and HCs was up to four‐fold (0.14 ± 0.04) that of PVMA and PVLA with hematopoietic HCs (0.033 ± 0.01). Moreover, cellular fluorescence increased significantly with increasing incubation time and increasing polymer concentration. Using hematopoietic lineage‐specific antibodies, cells were stained and analyzed by flow cytometry to confirm which HCs showed specific binding with glycopolymers, especially hematopoietic stem cells and progenitor cells (c‐kit+), B‐lymphocyte progenitor cells (B220+), monocyte cells (CD11b+), and erythrocytes (Ter119+). © 2007 Wiley Periodicals, Inc. J Biomed Mater Res 2007