Premium
Expression of core binding factor 1 and osteoblastic markers in C2C12 cells induced by calcium phosphate ceramics in vitro
Author(s) -
Tan Yanfei,
Wang Gang,
Fan Hongsong,
Wang Xinlong,
Lu Jian,
Zhang Xingdong
Publication year - 2007
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31125
Subject(s) - bone sialoprotein , materials science , osteocalcin , c2c12 , indian hedgehog , calcium , microbiology and biotechnology , in vitro , chondrocyte , biology , biochemistry , alkaline phosphatase , myogenesis , metallurgy , enzyme
The in vivo osteoinductive capacity of porous calcium phosphate ceramics (Ca/P ceramics) with special structure and phase composition had been found for almost decades. The mechanism of the osteoinductivity of porous calcium phosphate is studied by C2C12 cells culture in this paper. C2C12 cells were cocultured with four kinds of porous Ca/P ceramics for 2 and 5 days, without adding other growth factors. The four kinds of Ca/P ceramics were pure HA sintered at 1250°C and HA/TCP with a ratio of 60/40 sintered at 1100, 1200, and 1250°C respectively. RT‐PCR analysis found that the Ca/P ceramics induced the expression of Cbfa1, collagen type I, bone sialoprotein, and osteocalcin in C2C12 cells, while they did not induce mRNA expression of Indian hedgehog (IHH) that regulate chondrocyte differentiation. Our results showed that Ca/P ceramics alone were sufficient to induce C2C12 cells differentiation. The induction of bone‐related markers expression by Ca/P ceramics in osteoprogenitor cells suggested that the osteogenesis induced by the ceramics was intramembranous and the osteoinductivity was their intrinsic property. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007