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Shape‐engineered vascular endothelial cells: Nitric oxide production, cell elasticity, and actin cytoskeletal features
Author(s) -
Kidoaki Satoru,
Matsuda Takehisa
Publication year - 2007
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31112
Subject(s) - cytoskeleton , materials science , actin , stress fiber , elasticity (physics) , intracellular , actin cytoskeleton , biophysics , tissue engineering , cell , fluorescence microscope , nitric oxide , microbiology and biotechnology , biomedical engineering , chemistry , fluorescence , composite material , biology , biochemistry , optics , physics , medicine , organic chemistry
Single cell shape determines cellular functions. Therefore, control of cell shape is of considerable importance for the tissue engineering field. This study was designed to assess the effect of surface‐induced shaping of vascular endothelial cells (ECs) on the intracellular nitric oxide (NO) production level, the cell elasticity, and cytoskeletal (CSK) features on shape‐engineered ECs (round, 90, 120 μm diameter; spindle‐shaped, 20, 30, 40 μm width) prepared on a photolithographically microprocessed surface. Intracellular NO production was measured using a microscopic spectrometer with diaminofluorescein diacetate probe. Cell elasticity and actin CSK features were analyzed through microindentation measurement and fluorescence observations with fluorescence and atomic force microscopy. Results showed that spindle‐shaped cells exhibited lower NO production, higher cell stiffness, and denser actin stress fibers than the round and nonrestrictedly cultured control cells. Relations between cell shape with NO production, cell elasticity, and actin CSK features were discussed. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007