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LIF‐immobilized nonwoven polyester fabrics for cultivation of murine embryonic stem cells
Author(s) -
Çetinkaya Gaye,
Türkoğlu Hilal,
Arat Sezen,
Odaman Hande,
Onur Mehmet A.,
Gümüşderelioğlu Menemşe,
Tümer Aşkın
Publication year - 2007
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31107
Subject(s) - polyester , embryonic stem cell , materials science , alkaline phosphatase , leukemia inhibitory factor , tissue engineering , cell culture , scaffold , matrix (chemical analysis) , induced pluripotent stem cell , in vitro , biophysics , polymer chemistry , biomedical engineering , biochemistry , chemistry , biology , composite material , enzyme , medicine , genetics , gene
Embryonic stem (ES) cells have a great interest for tissue engineering because of their pluripotent nature and proliferative capacity. The objective of this study is to constitute a synthetic microenvironment to support the in vitro propagation of murine ES cells in an undifferentiated state. That is why we used a three‐dimensional matrix, nonwoven polyester fabric (NWPF), which was formed from poly(ethylene terephthalate) (PET) fibers. NWPF discs were partially hydrolyzed, and then the carboxyl groups were coupled with leukemia inhibitory factor (LIF) in the presence of water‐soluble carbodiimide. The effectiveness of immobilization process was checked with ATR‐FTIR spectroscopy, fluorimetry, and cell culture studies. ES cell colony morphology, alkaline phosphatase (AP) activity, stage‐specific embryonic antigen‐1 (SSEA‐1) immunoreactivity, and SEM analysis following a 72–96‐h culture period upon hydrolyzed and LIF‐immobilized surfaces were assessed to determine the pluripotent status of ES cells. Results revealed that LIF was active in immobilized form; undifferentiated colonies had not only a significant AP and SSEA‐1 immunoreactivity, but also a higher undifferentiated colony ratio on LIF‐immobilized surfaces than that of hydrolyzed surfaces. The immobilized LIF protein might be a good model to provide a feeder‐free system, but the physical properties of the scaffold is more convenient for differentiation studies. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007.