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Layer‐by‐layer assembly of biomacromolecules on poly(ethylene terephthalate) films and fiber fabrics to promote endothelial cell growth
Author(s) -
Liu Yunxiao,
He Tao,
Song Haiqing,
Gao Changyou
Publication year - 2006
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31102
Subject(s) - materials science , x ray photoelectron spectroscopy , extracellular matrix , layer by layer , layer (electronics) , scanning electron microscope , morphology (biology) , fiber , biophysics , chemical engineering , ethylene , chondroitin sulfate , adhesion , glycosaminoglycan , composite material , biochemistry , chemistry , biology , engineering , genetics , catalysis
Poly(ethylene terephthalate) (PET) film and PET vascular graft were modified by layer‐by‐layer (LBL) assembly of collagen and chondroitin sulfate (CS) to mimic the natural composition and structure of extracellular matrix. PET materials were first hydrolyzed to endow the surfaces with negative charge. Alternative assembly of positively charged collagen and negatively charged CS was then conducted in an LBL manner to produce biological multilayers. X‐ray photoelectron spectroscopy confirmed the occurrence of hydrolysis and existence of collagen and CS. The stepwise layer growth on the PET film was monitored by UV–vis spectroscopy. The multilayers had sufficient stability to pass through the cell culture period (8 days). In vitro endothelial cell culture revealed that the incorporated biomacromolecules could significantly improve the cell attachment, proliferation, and viability. Cells showed more spreading morphology with preserved phenotype, as illustrated by scanning electron microscopy and factor VIII‐related antigen immunocytochemistry, respectively. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res 80A:, 2007