Premium
Transfer of apatite coating from porogens to scaffolds: Uniform apatite coating within porous poly( DL ‐lactic‐ co ‐glycolic acid) scaffold in vitro
Author(s) -
Li Jiashen,
Beaussart Audrey,
Chen Yun,
Mak Arthur F.T.
Publication year - 2007
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31096
Subject(s) - materials science , apatite , plga , scaffold , coating , chemical engineering , porosity , glycolic acid , biocompatibility , interconnectivity , scanning electron microscope , bioceramic , composite material , nanoparticle , biomedical engineering , nanotechnology , lactic acid , metallurgy , medicine , artificial intelligence , biology , bacteria , computer science , engineering , genetics
Strategies to bone tissue engineering have focused on the use of synthetic or natural degradable materials as scaffolds for cell transplantation to guide bone regeneration. Biocompatibility, biodegradability, biomechanical integrity, and osteoconductivity are important requirements for the scaffold materials. This study explored a new approach of apatite coating to enhance the osteoconductivity of a synthetic degradable poly( DL ‐lactic‐ co ‐glycolic acid) (PLGA) scaffold. The new approach was developed to ensure a relatively uniform apatite coating on the interior pore surfaces deep inside a scaffold, even for a relatively thick scaffold with small pores. Apatite was first coated on the surface of paraffin spheres of the desirable sizes. The paraffin spheres were then molded to form a foam. PLGA/pyridine solution was cast into the interspaces among the paraffin spheres. After the paraffin spheres were dissolved and removed by cyclohexane, PLGA scaffold with controlled pore size, good interconnectivity and high porosity was obtained with apatite left on the pore surface uniformly throughout the whole scaffold. The scaffold and apatite coating were characterized using thermogravimetry analysis, scanning electron microscopy, energy dispersive X‐ray spectroscopy, and X‐ray diffractometry. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2007