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Characterization of a slowly degrading biodegradable polyesterurethane for tissue engineering scaffolds
Author(s) -
Henry Jerome A.,
Simonet Marc,
Pandit Abhay,
Neuenschwander Peter
Publication year - 2007
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31094
Subject(s) - materials science , differential scanning calorimetry , ultimate tensile strength , scanning electron microscope , tissue engineering , composite material , gel permeation chromatography , tensile testing , chemical engineering , polymer , electrospinning , morphology (biology) , biomedical engineering , medicine , physics , thermodynamics , engineering , genetics , biology
The purpose of this research was to develop and characterize a novel, slowly degrading polyesterurethane. In this study, a polyesterurethane with a crystalline segment of poly((R)‐3‐hydroxybutyric acid)‐diol linked by a diisocyanate to an amorphous segment of poly(ε‐caprolactone‐ co ‐glycolide)‐diol was synthesized. Porous and nonporous scaffolds were processed using electrospinning and solvent casting respectively. The morphology, pore size, and filament diameter of the mesh and film were characterized using scanning electron microscopy (SEM). The thermal properties were examined using differential scanning calorimetry (DSC). A degradation study was initiated to characterize the change in mechanical properties, molecular weight, and surface morphology over 12 months using tensile testing, gel permeation chromatography (GPC), and SEM respectively. Concomitantly, cell morphology and viability on these variants were investigated using fibroblasts. The mechanical test data indicated a gradual decrease in the ultimate tensile strength and strain to break while the modulus of elasticity remained stable. GPC data suggested a slow decrease in the molecular weight while SEM examination revealed changed surface morphologies. The in vitro studies implied that the novel poly‐esterurethane was not cytotoxic and that the mesh was a more favorable scaffold towards cell viability. The summation of these results suggests that this polyesterurethane has the potential for tissue engineering applications. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res 2007