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A novel methodology for imaging new bone formation around bioceramic bone substitutes
Author(s) -
Gorustovich Alejandro A.,
Sivak Matías G.,
Guglielmotti María B.
Publication year - 2007
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31071
Subject(s) - bioceramic , materials science , confocal , biomedical engineering , confocal microscopy , confocal laser scanning microscopy , bone tissue , composite material , optics , medicine , physics
In this study, Frost's bulk‐staining in combination with confocal laser scanning microscopy (CLSM) was used to image and characterize ground sections of undecalcified rat bone tissue with in situ bioceramic implants. This was addressed by bulk staining specimens in alcohol‐soluble basic fuchsin dye. The ground sections were imaged using CLSM in the confocal fluorescence mode. Confocal images revealed that the newly formed bone could be clearly distinguished from bone marrow and cortical bone, as well as from the implant material. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2007