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Fabrication and characterization of PLLA–chitosan hybrid scaffolds with improved cell compatibility
Author(s) -
Jiao Yanpeng,
Liu Zonghua,
Zhou Changren
Publication year - 2006
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31061
Subject(s) - chitosan , materials science , microporous material , scanning electron microscope , chemical engineering , tissue engineering , viability assay , composite material , biomedical engineering , cell , chemistry , medicine , biochemistry , engineering
To combine the individual advantages of synthetic and natural polymers, poly( L ‐lactic acid) (PLLA)–chitosan hybrid scaffolds were fabricated. PLLA sponges were prepared by particulate‐leaching, and then PLLA–chitosan hybrid scaffolds were obtained by dipping the PLLA sponges in chitosan solution and subsequently freeze–drying. Physicochemical properties of the scaffolds were characterized by scanning electron microscopy (SEM), water uptake test, and mechanical strength measurement. Moreover, cell adhesion, cell proliferation, and cell viability on the scaffolds were evaluated through osteoblast‐like cell culture. The experimental results indicated that, PLLA sponges exhibited macroporous structure and the interconnected microporous structure of chitosan was formed within the macropores of PLLA sponges. The incorporation of chitosan reinforced PLLA sponges in dependence on chitosan content. The hybrid scaffolds had higher water uptake ability compared with PLLA sponges. Particularly, the hybrid scaffolds exhibited excellent cell attachment efficiency, cell proliferation, and cell viability. This study suggests that the hybrid scaffolds obtain good mechanical strength from PLLA and excellent cell compatibility from chitosan. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006