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Developing an alginate/chitosan hybrid fiber scaffold for annulus fibrosus cells
Author(s) -
Shao Xinxin,
Hunter Christopher J.
Publication year - 2007
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31030
Subject(s) - scaffold , materials science , chitosan , tissue engineering , extracellular matrix , biomedical engineering , cell culture , fiber , aggrecan , intervertebral disc , spheroid , biophysics , anatomy , microbiology and biotechnology , composite material , chemistry , pathology , biology , medicine , biochemistry , genetics , articular cartilage , alternative medicine , osteoarthritis
Abstract A fibrous scaffold made of alginate or alginate/chitosan was fabricated for annulus fibrosus (AF) cell culture using a wet‐spinning and lyophilization technique. The scaffolds were evaluated using several in vitro tests. Scanning electron microscopy showed the scaffold fibers generally aligned in one direction with individual fiber diameters varying between 40–100 μm. The alginate/chitosan hybrid scaffold exhibited a slower degradation rate, while both scaffold types did not display any cyto‐toxicity to 3T3 fibroblasts and could maintain canine AF cell growth. The AF cells retained their spherical shape within the fibrous scaffold at the beginning of the culture period and formed into cell clusters at later times. Specific extracellular matrix molecules, including collagen I, collagen II, and aggrecan, could be detected in the AF cell clusters. These results demonstrate the feasibility of using this hybrid alginate/chitosan scaffold for AF cell culture, and the potential application for intervertebral disc tissue engineering. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res 2007