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Osteogenic differentiation of marrow stromal cells cultured on nanoporous alumina surfaces
Author(s) -
Popat Ketul C.,
Chatvanichkul KwanIsara,
Barnes George L.,
Latempa Thomas Joseph,
Grimes Craigs A.,
Desai Tejal A.
Publication year - 2006
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31028
Subject(s) - materials science , nanoporous , stromal cell , bone marrow , composite material , biomedical engineering , nanotechnology , cancer research , immunology , biology , medicine
Abstract A major goal in orthopedic biomaterials research is to design implant surfaces, which will enhance osseointegration in vivo . Several microscale as well as nanoscale architectures have been shown to significantly affect the functionality of bone cells i.e., osteoblasts. In this work, nanoporous alumina surfaces fabricated by a two‐step anodization process were used. The nanostructure of these surfaces can be controlled by varying the voltage used for anodization process. Marrow stromal cells were isolated from mice and seeded on nanoporous and amorphous (control) alumina surfaces. Cell adhesion, proliferation, and viability were investigated for up to 7 days of culture. Furthermore, the cell functionality was investigated by calcein staining. The cells were provided with differentiation media after 7 days of culture. The alkaline phosphatase (ALP) activity and matrix production were quantified using a colorimetric assay and X‐ray photoelectron spectroscopy (XPS) for up to 3 weeks of culture (2 weeks after providing differentiation media). Further, scanning electron microscopy (SEM) was used to investigate osteoblast morphology on these nanoporous surfaces. Over the 3‐week study, the nanoporous alumina surfaces demonstrated ∼45% increase in cell adhesion, proliferation, and viability, 35% increase in ALP activity, and 50% increase in matrix production when compared with the control surfaces. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006