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Optimization of the methods for introduction of amine groups onto the silica nanoparticle surface
Author(s) -
Liu Song,
Zhang HaiLi,
Liu TianCai,
Liu Bo,
Cao YuanCheng,
Huang ZhenLi,
Zhao YuanDi,
Luo QingMing
Publication year - 2007
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31026
Subject(s) - tetraethyl orthosilicate , amine gas treating , surface modification , materials science , carbodiimide , biomolecule , succinimide , nanoparticle , bioanalysis , silane , transferrin , combinatorial chemistry , polymer chemistry , chemical engineering , organic chemistry , nanotechnology , chemistry , biochemistry , engineering , composite material
The luminescent silica nanoparticle has attracted the researchers' concentration in bioanalysis recently. Its extensive application is based on the immobilization of various biomolecules such as deoxyribonucleic acid, antibody, and so forth onto the surface. By comparing different introduction methods of amine groups, it was confirmed that the “two‐step” route is more preferable by adding tetraethyl orthosilicate and 3‐aminopropyl‐(triethoxyl)silane in sequence, to attain ideal amine‐modified silica nanoparticles. On this basis, carboxyl groups were derived from amine groups on the nanoparticle surface and then were activated by 1‐ethyl‐3‐3‐(3‐dimethylaminopropyl) carbodiimide hydrochloride and N ‐hydroxy‐succinimide. Finally, mouse monoclonal antihuman CD71 antibody (McAb CD71) and transferrin were effectively linked with the carboxyl groups and successfully labeled the receptors in the membrane of fibroblast cells, respectively. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2007