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Calcium phosphate bone cements, functionalized with VEGF: Release kinetics and biological activity
Author(s) -
Lode A.,
WolfBrandstetter C.,
Reinstorf A.,
Bernhardt A.,
König U.,
Pompe W.,
Gelinsky M.
Publication year - 2007
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31024
Subject(s) - angiogenesis , vascular endothelial growth factor , materials science , calcium , chemistry , cancer research , vegf receptors , biology , metallurgy
Calcium phosphate bone cements are of great interest for bone replacement since the nanocrystalline structure allows their remodelling into native bone tissue. A strategy to accelerate vascularization of the implant region is the functionalization with vascular endothelial growth factor (VEGF), which is known to mediate angiogenesis in vivo . In this study, the release of recombinant human VEGF (rhVEGF 165 ) following physical adsorption to Biocement D (BioD) and several modifications were investigated. Our data demonstrate a high VEGF binding capacity of BioD and a sustained release with a moderate initial burst. A proliferation assay using endothelial cells revealed maintenance of biological activity of VEGF after release from BioD. Release behavior of BioD was not improved by modification with mineralized collagen type I, as well as with a combination of mineralized collagen with O ‐phospho‐ L ‐serine and sodium citrate, respectively. In contrast, a positive impact of these modifications on the activity of released VEGF was observed; in case of the phosphoserine‐ and sodium citrate‐modified cements, the biological efficacy of released VEGF was even higher than that of nonreleased control VEGF. We conclude that the bone implant material BioD and, especially, the phosphoserine modification may support activation of angiogenesis by delivery of VEGF in a local and sustained manner. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2007