Premium
Evaluation of laser spallation as a technique for measurement of cell adhesion strength
Author(s) -
Hagerman Elizabeth,
Shim Jaewoo,
Gupta Vijay,
Wu Ben
Publication year - 2007
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31011
Subject(s) - spallation , materials science , adhesion , biomaterial , cell adhesion , fibronectin , biophysics , polystyrene , laser , biomedical engineering , composite material , cell , nanotechnology , polymer , optics , chemistry , quantum mechanics , biology , neutron , medicine , biochemistry , physics
Cell adhesion to material surfaces is one of the fundamental phenomena of cellular response to implanted devices. Controlling the strength, dynamics, and mechanics of cell adhesion offer opportunities for designing novel biomaterials for tissue engineering and biotechnology. Many techniques have been developed for the purpose of quantifying various types of cell–biomaterial interaction. One method to evaluate cell affinity for a biomaterial is to measure the stress required to remove adherent cells from the material. This study investigates the possibility of using laser spallation, a technique previously developed for measuring the tensile strength of thin film interfaces, for evaluation of initial cell attachment strength. MC3T3‐E1 preosteoblasts were cultured on fibronectin‐coated polystyrene, a surface known to engage cells in receptor mediated adhesion, and untreated polystyrene, which elicit nonspecific adhesion mechanisms during early stages of cell attachment. The laser spallation technique effectively detached cells from polymer substrates and also distinguished relative cell adhesion strengths to surfaces with known differences in cell binding affinities. Scanning electron micrographs determined that cell detachment resulting from laser spallation left a cleaner surface than jet impingement, possibly suggesting a more complete detachment mechanism. Absolute values of adhesion strengths determined by laser spallation were significantly higher than those found using jet impingement, a previously reported hydrodynamic technique. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007