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Preclinical biocompatibility assessment of the EVAHEART ventricular assist device: Coating comparison and platelet activation
Author(s) -
Snyder Trevor A.,
Tsukui Hiroyuki,
Kihara Shin'Ichiro,
Akimoto Takehide,
Litwak Kenneth N.,
Kameneva Marina V.,
Yamazaki Kenji,
Wagner William R.
Publication year - 2007
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31006
Subject(s) - platelet , biocompatibility , platelet activation , biomedical engineering , materials science , biocompatible material , flow cytometry , medicine , implant , cd63 , surgery , immunology , biology , microrna , biochemistry , microvesicles , metallurgy , gene
Thromboembolism and bleeding remain significant complications of ventricular assist device (VAD) support. Increasing the amount of biocompatibility data collected during preclinical studies can provide additional criteria to evaluate device refinements, while design changes may be implemented before entering clinical use. Twenty bovines were implanted with the EVAHEART centrifugal VAD for durations from 30 to 196 days. Titanium alloy pumps were coated with either diamond‐like carbon or 2‐methoxyethyloylphosphoryl choline (MPC). Activated platelets and platelet microaggregates were quantified by flow cytometry, including two new assays to quantify bovine platelets expressing CD62P and CD63. Temporally, all assays were low preoperatively, then significantly increased following VAD implantation, before declining to a lower, but still elevated level over 2–3 weeks. MPC‐coated VADs produced significantly fewer activated platelets after implant trauma effects diminished. Three animals receiving no postoperative anticoagulation had similar amounts of circulating activated platelets and platelet microaggregates as animals receiving warfarin anticoagulation. Two new methods to quantify bovine activated platelets using antibodies to CD62P and CD63 were characterized and applied. These measures, along with previously described assays, were able to differentiate between two biocompatible coatings and assess effects of anticoagulation regimen in VAD preclinical testing. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2007