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Preparation and assessment of glutaraldehyde‐crosslinked collagen–chitosan hydrogels for adipose tissue engineering
Author(s) -
Wu Xuemei,
Black Lindsay,
SantacanaLaffitte Guido,
Patrick, Charles W.
Publication year - 2007
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31003
Subject(s) - glutaraldehyde , chitosan , adipose tissue , materials science , tissue engineering , self healing hydrogels , scaffold , biomedical engineering , in vivo , chemical engineering , polymer chemistry , chemistry , biochemistry , chromatography , microbiology and biotechnology , medicine , engineering , biology
A portfolio of crosslinked chitosan:collagen blends was prepared, and their microarchitecture and water binding capacity were studied to investigate their application for adipose tissue engineering. Glutaraldehyde (GA) concentration had little effect on scaffold morphology or water binding capacity. However, the processing freezing temperature prior to lyophilization affected both. In vitro cytocompatibility of preadipocytes (PAs) was assessed for a candidate collagen:chitosan blend using two assays. Results confirm the viability of PAs on GA‐crosslinked collagen:chitosan scaffolds. A rat subcutaneous pocket assay was employed to assess PA‐seeded scaffolds in vivo . Animal tests proved that PA‐seeded scaffolds were biocompatible, could induce vascularization, and form adipose tissue. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006