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Functionalized hydrogel surfaces for the patterning of multiple biomolecules
Author(s) -
Hynd Matthew R.,
Frampton John P.,
Burnham MaryRose,
Martin David L.,
DowellMesfin Natalie M.,
Turner James N.,
Shain William
Publication year - 2007
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31002
Subject(s) - streptavidin , biomolecule , materials science , biotinylation , fluorophore , polydimethylsiloxane , self healing hydrogels , nanotechnology , nanoporous , protein microarray , soft lithography , fluorescence , biotin , chemistry , biochemistry , polymer chemistry , medicine , microarray , physics , gene expression , alternative medicine , pathology , quantum mechanics , fabrication , gene
Patterning of multiple proteins and enzymes onto biocompatible surfaces can provide multiple signals to control cell attachment and growth. Acrylamide‐based hydrogels were photo‐polymerized in the presence of streptavidin–acrylamide, resulting in planar gel surfaces functionalized with the streptavidin protein. This surface was capable of binding biotin‐labeled biomolecules. The proteins fibronectin and laminin, the enzyme alkaline phosphatase, and the photo‐protein R‐phycoerythrin were patterned using soft lithographic techniques. Polydimethylsiloxane stamps were used to transfer biotinylated proteins onto streptavidin‐conjugated hydrogel surfaces. Stamped biomolecules were spatially resolved to feature sizes of 10 μm. Fluorescence measurements were used to assess protein transfer and enzyme functionality on modified surfaces. Our results demonstrate that hydrogel surfaces can be patterned with multiple proteins and enzymes, with retention of biological and catalytic activity. These surfaces are biocompatible and provide cues for cell attachment and growth. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res 2007