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Distinctin improves the efficacies of glycopeptides and betalactams against staphylococcal biofilm in an experimental model of central venous catheter infection
Author(s) -
Giacometti Andrea,
Cirioni Oscar,
Ghiselli Roberto,
Orlando Fiorenza,
Silvestri Carmela,
Renzone Giovanni,
Testa Irma,
Mocchegiani Federico,
Vittoria Agnese Della,
Saba Vittorio,
Scaloni Andrea,
Scalise Giorgio
Publication year - 2007
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30982
Subject(s) - antibiotics , in vivo , central venous catheter , staphylococcus aureus , biofilm , bacteremia , microbiology and biotechnology , catheter , in vitro , bloodstream infection , vancomycin , micrococcaceae , bacteria , medicine , pharmacology , surgery , antibacterial agent , biology , biochemistry , genetics
The ability of microorganisms to adhere to medical implants is a problem of high visibility and has been focused in numerous investigations. To assess the efficacy of distinctin and conventional antibiotics in the treatment of central venous catheter in vitro and in vivo studies were performed. The in vitro susceptibility assay was performed against S. aureus biofilms developed on 96‐well polystyrene tissue culture plates. Efficacy studies were performed in a rat model of CVC infection. Twenty‐four hours after implantation, the catheters were filled with distinctin. Thirty minutes later, rats were challenged via the CVC with S. aureus . Administration of antibiotics into the CVC at a concentration equal to the MBC for adherent cells, or at 1024 μg/mL began 24 h later. The killing activities of all antibiotics against adherent bacteria were at least four‐ to eightfold lower than against freely growing cells. When antibiotics were used in distinctin pretreated wells, they showed a significant increase of activity. The in vivo studies showed that when CVCs were pretreated with distinctin biofilm bacterial load was further decreased to 10 1 CFU/mL and bacteremia was not detected. Distinctin displays potential as an adjunctive agent to antibiotics in the treatment of CVC‐related infections. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res 2007