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Three‐dimensional collagen fiber remodeling by mesenchymal stem cells requires the integrin–matrix interaction
Author(s) -
Chang ChingFang,
Lee MingWei,
Kuo PeiYin,
Wang YngJiin,
Tu YiHsien,
Hung ShihChieh
Publication year - 2007
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30963
Subject(s) - mesenchymal stem cell , materials science , chondrogenesis , biomaterial , cartilage , tissue engineering , microbiology and biotechnology , integrin , matrix (chemical analysis) , cell adhesion , stem cell , biomedical engineering , type ii collagen , cell , adhesion , nanotechnology , anatomy , chemistry , biology , composite material , biochemistry , medicine
Tissue engineering aiming to repair or regenerate damaged tissues necessitates fabricating three‐dimensional biomaterial scaffolds with controlled porosity for delivering cells. To facilitate cell distribution, a strategy using stem cell‐based fabrication of biomaterials was tested in type II collagen fibers. Human mesenchymal stem cells when delivered in type II collagen assembled and reorganized these matrices and differentiated into spherical chondrocytes with the synthesis of cartilage proteins. The cell‐mediated assembly and reorganization of collagen fibers was not limitless and only restricted to an appropriate ratio of cell number and collagen amount. The blocking of α2 or β1‐integrin function with specific antibodies significantly impeded the collagen‐assembly effects. In vitro chondrogenesis or in vivo cartilage formation of human mesenchymal stem cells was also dependent on the interactions between cells and surrounding matrices. This method for three‐dimensional fabricating collagen fibers may generally be applied to other biomaterials, when combined with surface modification or ligand addition for cell adhesion. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2007