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Glow discharge plasma treatment of polyethylene tubing with tetraglyme results in ultralow fibrinogen adsorption and greatly reduced platelet adhesion
Author(s) -
Cao Lan,
Sukavaneshvar Sivaprasad,
Ratner Buddy D.,
Horbett Thomas A.
Publication year - 2006
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30908
Subject(s) - materials science , platelet , ex vivo , platelet adhesiveness , platelet activation , adhesion , fibrinogen , adsorption , in vivo , protein adsorption , polymer , composite material , in vitro , chemistry , biochemistry , organic chemistry , immunology , medicine , platelet aggregation , biology , microbiology and biotechnology
Abstract Previous studies from our lab have shown that fibrinogen adsorption (Γ Fg ) must be reduced below 10 ng/cm 2 to significantly reduce platelet adhesion, and that radio frequency glow discharge (RFGD) treatment of polymeric films in the presence of tetraethylene glycol dimethyl ether (tetraglyme) can reduce Γ Fg to the desired ultralow value. In this report, the effects of RFGD coatings of tetraglyme on the lumenal surface of PE tubing on Γ Fg and on blood interactions both in vitro and ex vivo are described. Γ Fg on the tetraglyme‐coated PE tubing was reduced to the desired ultralow level (<10 ng/cm 2 ), and we also observed a significant decrease in adsorption of von Willebrand's factor. In vitro platelet adhesion from washed platelet suspensions, platelet rich plasma, or whole blood to tetraglyme‐coated PE tubing was decreased compared to PE, polyurethane, or silicone rubber tubes. In addition, thrombin generation by platelets adherent to tetraglyme‐coated PE was also much less than by platelets adherent to PE. When inserted in an ex vivo carotid artery‐carotid artery shunt in sheep, the RFGD tetraglyme‐coated PE exhibited a very low number of adherent platelets compared to heparin‐coated, chromic acid‐etched, or plain PE. The RFGD tetraglyme‐coated PE tubes exhibited high protein and platelet resistance in vitro, and high platelet resistance ex vivo . The improved hemocompatibility is attributed to the unique chemical structure of RFGD tetraglyme that makes it highly protein resistant. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006