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Low‐intensity ultrasound stimulates the viability and matrix gene expression of human articular chondrocytes in alginate bead culture
Author(s) -
Choi Byung Hyune,
Woo JeongIm,
Min ByoungHyun,
Park So Ra
Publication year - 2006
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30816
Subject(s) - materials science , bead , matrix (chemical analysis) , ultrasound , gene expression , biomedical engineering , gene , composite material , biology , medicine , genetics , radiology
We investigated the effects of low‐intensity ultrasound (LIUS) on the activity of human articular chondrocytes isolated from osteoarthritis patients and cultured in the three‐dimensional alginate beads. LIUS was treated at 0, 100, 200, and 300 mW/cm 2 for 10 min everyday for 2, 7, or 15 days. LIUS induced the viability of cells only at day 15 but not until day 7 after treatment, when examined by trypan blue exclusion and LIVE/DEAD® assay kit. When examined at day 7, the proliferation of cells was not changed by LIUS in the 3 H‐thymine incorporation. The expression of matrix producing proteins (type II collagen and proteoglycan) was clearly induced by 200–300 mW/cm 2 LIUS in the incorporation of radioactivity and Northern blot analysis. Although the expression of MMP‐1, a matrix degrading protein, was decreased, that of TIMP‐1, an inhibitor of MMPs, was not affected by LIUS. Histological analysis revealed an increase in the number and size of glycosaminoglycan‐positive lacunae and cellular organelles, appearing as rough endoplasmic reticulum and mitochondria by LIUS. These results showed that the viability and metabolism of human articular chondrocytes in alginate culture was induced by LIUS treatment, suggesting that they could be a promising autologous source for cartilage tissue engineering. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006